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Image Search Results
Journal: Cancer Reports
Article Title: TAZ upregulates MIR‐224 to inhibit oxidative stress response in multiple myeloma
doi: 10.1002/cnr2.1879
Figure Lengend Snippet: TAZ represses the oxidative stress‐resistant phenotype in MM. (A) Immunoblot of DELTA47 cells following CRISPR‐mediated TAZ knockout, β‐actin was used as a loading control. (B) Relative mRNA expression of selected TAZ target genes (ANKRD1, CTGF, and CYR61). rRNA as the reference gene. (C) Cell viability of KO‐TAZ DELTA47 compared to DELTA47‐pLENTI following exposure to varying concentrations of H 2 O 2 in the cell culture system. Assessment of GSH/GSSG ratio in (D) KO‐TAZ and DELTA47‐pLENTI cells. (E) TAZ‐KMS27 and WPI‐KMS27 cells using a luminescence‐based assay. (F) Assessment of ROS generation using DCFDA assay in KO‐TAZ and DELTA47‐pLENTI cells.
Article Snippet: Intracellular ROS generation was determined using the
Techniques: Western Blot, CRISPR, Knock-Out, Control, Expressing, Cell Culture, Luminescence Assay