dcfh da Search Results


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Cayman Chemical dichloro dihydro fluorescein diacetate (dcfda) assay kit
TAZ represses the oxidative stress‐resistant phenotype in MM. (A) Immunoblot of DELTA47 cells following CRISPR‐mediated TAZ knockout, β‐actin was used as a loading control. (B) Relative mRNA expression of selected TAZ target genes (ANKRD1, CTGF, and CYR61). rRNA as the reference gene. (C) Cell viability of KO‐TAZ DELTA47 compared to DELTA47‐pLENTI following exposure to varying concentrations of H 2 O 2 in the cell culture system. Assessment of GSH/GSSG ratio in (D) KO‐TAZ and DELTA47‐pLENTI cells. (E) TAZ‐KMS27 and WPI‐KMS27 cells using a luminescence‐based assay. (F) Assessment of ROS generation using <t>DCFDA</t> assay in KO‐TAZ and DELTA47‐pLENTI cells.
Dichloro Dihydro Fluorescein Diacetate (Dcfda) Assay Kit, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing Solarbio Science dcfh-da
TAZ represses the oxidative stress‐resistant phenotype in MM. (A) Immunoblot of DELTA47 cells following CRISPR‐mediated TAZ knockout, β‐actin was used as a loading control. (B) Relative mRNA expression of selected TAZ target genes (ANKRD1, CTGF, and CYR61). rRNA as the reference gene. (C) Cell viability of KO‐TAZ DELTA47 compared to DELTA47‐pLENTI following exposure to varying concentrations of H 2 O 2 in the cell culture system. Assessment of GSH/GSSG ratio in (D) KO‐TAZ and DELTA47‐pLENTI cells. (E) TAZ‐KMS27 and WPI‐KMS27 cells using a luminescence‐based assay. (F) Assessment of ROS generation using <t>DCFDA</t> assay in KO‐TAZ and DELTA47‐pLENTI cells.
Dcfh Da, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson dcfh-da
TAZ represses the oxidative stress‐resistant phenotype in MM. (A) Immunoblot of DELTA47 cells following CRISPR‐mediated TAZ knockout, β‐actin was used as a loading control. (B) Relative mRNA expression of selected TAZ target genes (ANKRD1, CTGF, and CYR61). rRNA as the reference gene. (C) Cell viability of KO‐TAZ DELTA47 compared to DELTA47‐pLENTI following exposure to varying concentrations of H 2 O 2 in the cell culture system. Assessment of GSH/GSSG ratio in (D) KO‐TAZ and DELTA47‐pLENTI cells. (E) TAZ‐KMS27 and WPI‐KMS27 cells using a luminescence‐based assay. (F) Assessment of ROS generation using <t>DCFDA</t> assay in KO‐TAZ and DELTA47‐pLENTI cells.
Dcfh Da, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


TAZ represses the oxidative stress‐resistant phenotype in MM. (A) Immunoblot of DELTA47 cells following CRISPR‐mediated TAZ knockout, β‐actin was used as a loading control. (B) Relative mRNA expression of selected TAZ target genes (ANKRD1, CTGF, and CYR61). rRNA as the reference gene. (C) Cell viability of KO‐TAZ DELTA47 compared to DELTA47‐pLENTI following exposure to varying concentrations of H 2 O 2 in the cell culture system. Assessment of GSH/GSSG ratio in (D) KO‐TAZ and DELTA47‐pLENTI cells. (E) TAZ‐KMS27 and WPI‐KMS27 cells using a luminescence‐based assay. (F) Assessment of ROS generation using DCFDA assay in KO‐TAZ and DELTA47‐pLENTI cells.

Journal: Cancer Reports

Article Title: TAZ upregulates MIR‐224 to inhibit oxidative stress response in multiple myeloma

doi: 10.1002/cnr2.1879

Figure Lengend Snippet: TAZ represses the oxidative stress‐resistant phenotype in MM. (A) Immunoblot of DELTA47 cells following CRISPR‐mediated TAZ knockout, β‐actin was used as a loading control. (B) Relative mRNA expression of selected TAZ target genes (ANKRD1, CTGF, and CYR61). rRNA as the reference gene. (C) Cell viability of KO‐TAZ DELTA47 compared to DELTA47‐pLENTI following exposure to varying concentrations of H 2 O 2 in the cell culture system. Assessment of GSH/GSSG ratio in (D) KO‐TAZ and DELTA47‐pLENTI cells. (E) TAZ‐KMS27 and WPI‐KMS27 cells using a luminescence‐based assay. (F) Assessment of ROS generation using DCFDA assay in KO‐TAZ and DELTA47‐pLENTI cells.

Article Snippet: Intracellular ROS generation was determined using the Dichloro‐dihydro‐fluorescein diacetate (DCFDA) assay kit (Cayman Chemical, USA) according to the manufacturer's instructions.

Techniques: Western Blot, CRISPR, Knock-Out, Control, Expressing, Cell Culture, Luminescence Assay